Recombinant Bovine bFGF Protein (animal-free)

Alternate Names

bFGF; FGF basic; FGF2; FGF-2; Fibroblast Growth Factor basic; basic fibroblast growth factor bFGF; Basic fibroblast growth factor; FGFBprostatropin; fibroblast growth factor 2 (basic); HBGF-2; heparin-binding growth factor 2; Prostatropin.

Characteristics

Species

Bovine

Expression System

E. coli

Fusion Tag

Tag Free

Protein Accession

P03969, Bovine bFGF (P10-S155)

Predicted Molecular Mass

16.5 kDa

Specification

Appearance

White powder, colorless clear liquid after reconstitution

Purity

≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)

Endotoxin Level

≤200 EU/mg by the LAL method

Biological Activity

Measured in a cell proliferation assay using NIH3T3 mouse embryonic fibroblast cells. The ED50 for this effect is ≤2.0 ng/mL.

Formulation

Lyophilized from a 0.22 μm-filtered solution containing 10 mM PB, 250 mM NaCl, 5% mannitol and 0.01% Tween 80, pH 7.4

Size

50 μg/vial, 1 mg/vial

Handling and Storage

Reconstitution

It is recommended to redissolve in sterile deionized water.

Shipping

The product is shipped with blue ice.

Storage & Stability

36 months at -20°C to -80°C in lyophilized state

6 months at -20°C to -80°C under sterile conditions after reconstitution

7-10 days at 2°C to 8°C under sterile conditions after reconstitution.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.