Recombinant Bovine Fetuin A Protein (animal-free)

Alternate Names

Fetuin A; Fetuin-A; FETUA; A2HS; AHS; AHSG; HSGA; Alpha-2-HS-Glycoprotein; Alpha-2-Z-Globulin; Ba-Alpha-2-Glycoprotein.

Characteristics

Species

Bovine

Expression System

Chinese Hamster Ovary (CHO) cell line

Fusion Tag

Tag Free

Protein Accession

P12763, Bovine Fetuin A (I19-I359)

Predicted Molecular Mass

36.4 kDa

Specification

Appearance

White powder, colorless clear liquid after reconstitution

Purity

≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)

Endotoxin Level

≤0.5 EU/mg by the LAL method

Biological Activity

Measured in a cell proliferation assay using B16-F1 cells. Recombinant Bovine Fetuin A (Cat. No. Y07511) stimulates adhesion of B16-F1 cells. The ED50 for this effect is ≤100 μg/mL. Optimal concentration depends on cell type as well as the application or research objectives.

Formulation

Lyophilized from a 0.22 μm-filtered solution containing 10 mM PB, pH 7.4

Size

100 mg/vial, 1g/vial, 10g/vial, 100g/vial

Handling and Storage

Reconstitution

It is recommended to redissolve in sterile deionized water.

Shipping

The product is shipped with blue ice.

Storage & Stability

36 months at -20°C to -80°C in lyophilized state

6 months at -20°C to -80°C under sterile conditions after reconstitution

7-10 days at 2°C to 8°C under sterile conditions after reconstitution.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.