Recombinant Human FLT3 Ligand Protein (animal-free)
Recombinant Human FLT3 Ligand Protein
Alternate Names
Flt3 Ligand; Flt3L; FLT3LG; Fms-Felated Tyrosine Kinase 3 Ligand; SL Cytokine.
Characteristics
Species
Human
Expression System
Chinese Hamster Ovary (CHO) cell line
Fusion Tag
Tag Free
Protein Accession
P49771, Human FLT-3L (Thr27-Pro184)
Predicted Molecular Mass
17.8 kDa
Specification
Appearance
White powder, colorless clear liquid after reconstitution
Purity
≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
Endotoxin Level
≤10 EU/mg by the LAL method
Biological Activity
The ED50 as determined by the dose-dependent stimulation of the proliferation of human OCI-AML5 cells is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥1 x 106 units/mg.
Formulation
Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% mannitol and 0.01% Tween 80, pH 7.4
Size
10 μg/vial, 50 μg/vial, 1 mg/vial
Handling and Storage
Reconstitution
It is recommended to redissolve in sterile deionized water.
Shipping
The product is shipped with blue ice.
Storage & Stability
36 months at -20°C to -80°C in lyophilized state
6 months at -20°C to -80°C under sterile conditions after reconstitution
7-10 days at 2°C to 8°C under sterile conditions after reconstitution.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.