Recombinant Human GH Protein (animal-free)

Alternate Names

GH1; Somatotropin; Growth hormone; GH; GH-N; Growth hormone 1; Pituitary growth hormone.

Characteristics

Species

Human

Expression System

Chinese Hamster Ovary (CHO) cell line

Fusion Tag

Tag Free

Protein Accession

P01241, Human GH (Phe27-Phe217)

Predicted Molecular Mass

22 kDa

Specification

Appearance

White powder, colorless clear liquid after reconstitution

Purity

≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)

Endotoxin Level

≤10 EU/mg by the LAL method

Biological Activity

Determined by its ability to stimulate the proliferation of Nb2-11 cells. Expected ED50 ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.

Formulation

Lyophilized from a 0.22 μm-filtered solution containing 10 mM PB

Size

10 μg/vial, 50 μg/vial, 1 mg/vial

Handling and Storage

Reconstitution

It is recommended to redissolve in sterile deionized water.

Shipping

The product is shipped with blue ice.

Storage & Stability

36 months at -20°C to -80°C in lyophilized state

6 months at -20°C to -80°C under sterile conditions after reconstitution

7-10 days at 2°C to 8°C under sterile conditions after reconstitution.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.