Recombinant Human HGF Protein (animal-free)

Alternate Names

HGF; Hepatocyte Growth Factor, deafness, autosomal recessive 39; DFNB39; EC 3.4.21; EC 3.4.21.7; fibroblast-derived tumor cytotoxic factor; F-TCF; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatopoeitin-A; Hepatopoietin A; HGF; HGFB; HPTA; HPTAhepatocyte growth factor; lung fibroblast-derived mitogen; Scatter factor; SF; SFhepatopoeitin-A.

Characteristics

Species

Human

Expression System

Chinese Hamster Ovary (CHO) cell line

Fusion Tag

Tag Free

Protein Accession

P14210, Human HGF (α chain: Gln32-Arg494, β chain: Val495-Ser728)

Predicted Molecular Mass

53.7 kDa (α chain), 26 kDa (β chain)

Specification

Appearance

White powder, colorless clear liquid after reconstitution

Purity

≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)

Endotoxin Level

≤10 EU/mg by the LAL method

Biological Activity

Measured in a cell proliferation assay using monkey 4MBr5 cells. The ED50 for this effect ≤20 ng/mL.

Formulation

Lyophilized from a 0.22 μm-filtered solution containing 10 mM PB, 250 mM NaCl, 5% mannitol and 0.01% Tween 80, pH 7.4

Size

10 μg/vial, 50 μg/vial, 1 mg/vial

Handling and Storage

Reconstitution

It is recommended to redissolve in sterile deionized water.

Shipping

The product is shipped with blue ice.

Storage & Stability

36 months at -20°C to -80°C in lyophilized state

6 months at -20°C to -80°C under sterile conditions after reconstitution

7-10 days at 2°C to 8°C under sterile conditions after reconstitution.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.