Recombinant Human IFN-alpha2b Protein (animal-free)
Recombinant Human IFN-alpha2b Protein
Alternate Names
IFN-alpha2b; interferon alpha 2b; Interferon Alpha-2; IFN-Alpha-2; Interferon Alpha-A; LeIF A; IFNA2.
Characteristics
Species
Human
Expression System
Chinese Hamster Ovary (CHO) cell line
Fusion Tag
Tag Free
Protein Accession
P01563, Human IFN-α2b (Cys24-Glu188)
Predicted Molecular Mass
19.2 kDa
Specification
Appearance
White powder, colorless clear liquid after reconstitution
Purity
≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
Endotoxin Level
≤10 EU/mg by the LAL method
Biological Activity
Measured in a VSV inhibition assay with Wish cells. The ED50 this effect is ≤100 pg/mL, corresponding to a specific activity is ≥1 ×10^7 units/mg.
Formulation
Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% mannitol and 0.01% Tween 80, pH 7.4
Size
50 μg/vial, 1 mg/vial
Handling and Storage
Reconstitution
It is recommended to redissolve in sterile deionized water.
Shipping
The product is shipped with blue ice.
Storage & Stability
36 months at -20°C to -80°C in lyophilized state
6 months at -20°C to -80°C under sterile conditions after reconstitution
7-10 days at 2°C to 8°C under sterile conditions after reconstitution.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.