Recombinant Human IL-18 Protein (animal-free)

Alternate Names

Interleukin-18; IL-18; IL18; Iboctadekin; IL-1F4; IL1F4; Interferon γ-inducing factor; IFN-γ-inducing factor; Interleukin-1 γ; IL-1 γ; GIF; IL-1g; MGC12320.

Characteristics

Species

Human

Expression System

Chinese Hamster Ovary (CHO) cell line

Fusion Tag

Tag Free

Protein Accession

Q14116, Human IL-18 (Tyr37-Asp193)

Predicted Molecular Mass

18.2 kDa

Specification

Appearance

White powder, colorless clear liquid after reconstitution

Purity

≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)

Endotoxin Level

≤10 EU/mg by the LAL method

Biological Activity

Measured by its ability to induce IFN-gamma secretion by KG‑1 human acute myelogenous leukemia cells in the presence of TNF-α. The ED50 for this effect is ≤2.0 ng/mL.

Formulation

Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% mannitol and 0.01% Tween 80, pH 7.4

Size

10 μg/vial, 50 μg/vial, 1 mg/vial

Handling and Storage

Reconstitution

It is recommended to redissolve in sterile deionized water.

Shipping

The product is shipped with blue ice.

Storage & Stability

36 months at -20°C to -80°C in lyophilized state

6 months at -20°C to -80°C under sterile conditions after reconstitution

7-10 days at 2°C to 8°C under sterile conditions after reconstitution.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.