Recombinant Human IL-3 Protein (animal-free)
Recombinant Human IL-3 Protein (animal-free)
Alternate Names
Interleukin-3; IL-3; IL3; Hematopoietic Growth Factor; Mast Cell Growth Factor; MCGF; Multipotential Colony-Stimulating Factor; P-Cell-Stimulating Factor.
Characteristics
Species
Human
Expression System
Chinese Hamster Ovary (CHO) cell line
Fusion Tag
Tag Free
Protein Accession
P08700, Human IL-3 (Ala20-Phe152)
Predicted Molecular Mass
15 kDa
Specification
Appearance
White powder, colorless clear liquid after reconstitution
Purity
≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
Endotoxin Level
≤10 EU/mg by the LAL method
Biological Activity
Measured in a cell proliferation assay using TF-1 human erythroleukemiccells. The ED50 for this effect is 0.02-0.1 ng/mL.
Formulation
Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% mannitol and 0.01% Tween 80, pH 7.4
Size
10 μg/vial, 50 μg/vial, 1 mg/vial
Handling and Storage
Reconstitution
It is recommended to redissolve in sterile deionized water.
Shipping
The product is shipped with blue ice.
Storage & Stability
36 months at -20°C to -80°C in lyophilized state
6 months at -20°C to -80°C under sterile conditions after reconstitution
7-10 days at 2°C to 8°C under sterile conditions after reconstitution.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.