Recombinant Human LIF Protein (animal-free)
Recombinant Human LIF Protein (animal-free)
Alternate Names
Leukemia Inhibitory Factor; LIF; Differentiation-Stimulating Factor; D Factor; Melanoma-Derived LPL Inhibitor; MLPLI; Emfilermin; LIF; HILDA.
Characteristics
Species
Human
Expression System
Chinese Hamster Ovary (CHO) cell line
Fusion Tag
Tag Free
Protein Accession
P15018, Human LIF (Ser23-Phe202)
Predicted Molecular Mass
19.7 kDa
Specification
Appearance
White powder, colorless clear liquid after reconstitution
Purity
≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
Endotoxin Level
≤10 EU/mg by the LAL method
Biological Activity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is ≤ 2 ng/mL. Formulation
Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% mannitol and 0.01% Tween 80, pH 7.4
Size
10 μg/vial, 50 μg/vial, 1 mg/vial
Handling and Storage
Reconstitution
It is recommended to redissolve in sterile deionized water.
Shipping
The product is shipped with blue ice.
Storage & Stability
36 months at -20°C to -80°C in lyophilized state
6 months at -20°C to -80°C under sterile conditions after reconstitution
7-10 days at 2°C to 8°C under sterile conditions after reconstitution.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.