Recombinant Human PDGF-AA Protein
Recombinant Human PDGF-AA Protein
Alternate Names: Platelet-Derived Growth Factor-AA, Glioma-derived growth factor (GDGF), Osteosarcoma-derived Growth Factor (ODGF)
Construction: A DNA sequence encoding the human PDGF-AA (NP_002598.4) was expressed with the C-terminal fused Fc region of human IgG1.
Expression Host: HEK293 cells
QC Testing Purity: > 95 % as determined by SDS-PAGE
Bio Activity: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by Balb/c 3T3 cells is ≤ 2 ng/ml
Endotoxin: < 0.01 EU per μg of the protein as determined by the LAL method
Molecular Mass: The recombinant human PDGF-AA consists of 358 amino acids and predicts a molecular mass of 41.2 kDa.
Formulation: Lyophilized from sterile PBS, pH 7.4. Normally 6-8 % trehalose and mannitol are added as protectants before lyophilization.
Storage: Store at -20°C. Aliquot to avoid repeated freeze/thaw cycles.
Producer: G&W Biotechnologie GmbH (http://www.gwbioth.com/)
Safety: For research use only. Not for use in human or clinical diagnosis.
Background
PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs, PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types, including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet α-granules, and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β. PDGFR-α is a high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGF-BB and PDGF-AB.
References
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