Recombinant Human PDGF-BB Protein

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Specification

Product name	Human PDGF-BB Protein
Sequence	Amino acid sequence derived from Human PDGF-BB (Ser82-Thr190) (P01127) was expressed.
Activity	Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 4-20 ng/ml.
Protein length	The recombinant Human PDGF-BB consists of 109 amino acids and predicts a molecular mass of 12.3 kDa.
Preparation method	E. coli
Purity	> 98 % as determined by SDS-PAGE
Alternative	PDGF-BB

 

Product Properties

Formulation	Lyophilized from PBS.
Features & Benefits	<1 EU per μg of the protein by the LAL method.
Molecular weight	12.3 kDa
Usage notes	Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized protein using the buffer we provided not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Storage instructions	Lyophilized Human PDGF-BB Protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Shipping	Gel pack with blue ice.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

 

Additional Information

Background	Platelet derived growth factor (PDGF) is a potent mitogen and chemoattractant for mesenchymal and osteogenic cells and stimulates angiogenic molecules which play an essential role in bone regeneration. PDGF-BB is a member of PDGF family, which promotes cell proliferation, survival and migration, through binding to the tyrosine kinase PDGF receptor.
Gene ID	5155
Alternative	PDGF-BB
Accession	P01127