Recombinant Human TGF-beta1 Protein (animal-free, DMF-040434)

Alternate Names

Transforming Growth Factor Beta-1; TGF-Beta1; TGF-β1; Latency-Associated Peptide; LAP; TGFB1; TGFB.

Characteristics

Species

Human

Expression System

Chinese Hamster Ovary (CHO) cell line

Fusion Tag

Tag Free

Protein Accession

P01137, Human TGF-β1 (Ala279-Ser390)

Predicted Molecular Mass

12.8 kDa (monomer)

Specification

Appearance

White powder, colorless clear liquid after reconstitution

Purity

≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)

Endotoxin Level

≤10 EU/mg by the LAL method

Biological Activity

Measured in a cell inhibition assay using TF-1 cells at IL4 presence. TheED50 for this effect is ≤0.1ng/mL.

Formulation

Lyophilized from a 0.22 μm-filtered solution containing 0.085% TFA, 30% ACN, 5% mannitol, pH 2.5

Size

10 μg/vial, 50 μg/vial, 1 mg/vial

Handling and Storage

Reconstitution

It is recommended to redissolve in 4 mM HCL.

Shipping

The product is shipped with blue ice.

Storage & Stability

36 months at -20°C to -80°C in lyophilized state

6 months at -20°C to -80°C under sterile conditions after reconstitution

7-10 days at 2°C to 8°C under sterile conditions after reconstitution.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.