Recombinant Human Transferrin (holo) Protein (animal-free)
Recombinant Human Transferrin (holo) Protein
Alternate Names
Holo Transferrin; Holo-Transferrin; Serotransferrin; Transferrin; Beta-1 metal-binding globulin; Siderophilin; TF.
Characteristics
Species
Human
Expression System
Chinese Hamster Ovary (CHO) cell line
Fusion Tag
Tag Free
Protein Accession
P02787, Human Transferrin (Val20-Pro698)
Predicted Molecular Mass
75.2 kDa
Specification
Appearance
Red powder, red clear liquid after reconstitution
Purity
≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
Endotoxin Level
≤0.5 EU/mg by the LAL method
Biological Activity
Measured in a serum-free cell proliferation assay using MDCK canine kidney epithelial cells. The ED50 for this effect is 0.5-2 μg/mL. Optimal concentration depends on cell type as well as the application or research objectives.
Formulation
Lyophilized from a 0.2 μm-filtered solution in sterile water
Size
100 mg/vial, 1 g/vial, 10 g/vial, 100 g/vial
Handling and Storage
Reconstitution
It is recommended to redissolve in sterile deionized water.
Shipping
The product is shipped with blue ice.
Storage & Stability
36 months at -20°C to -80°C in lyophilized state
6 months at -20°C to -80°C under sterile conditions after reconstitution
7-10 days at 2°C to 8°C under sterile conditions after reconstitution.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.